Recombinant Human Fibroblast growth factor 21 protein(FGF21) (Active)

Recombinant Human Fibroblast growth factor 21 protein(FGF21) (Active)

CSB-AP002471HU
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Size:100ug. Other sizes are also available. Please contact us.

Research Areas:Cancer

Uniprot NO.:Q9NSA1

Uniprot Entry Name:FGF21_HUMAN

Gene Names:FGF21,UNQ3115/PRO10196

Species:Homo sapiens (Human)

Source:E.Coli

Expression Region:29-209aa

Sequence:HPIPDSSPLL QFGGQVRQRY LYTDDAQQTE AHLEIREDGT VGGAADQSPE SLLQLKALKP GVIQILGVKT SRFLCQRPDG ALYGSLHFDP EACSFRELLL EDGYNVYQSE AHGLPLHLPG NKSPHRDPAP RGPARFLPLP GLPPALPEPP GILAPQPPDV GSSDPLSMVG PSQGRSPSYA S

Protein Description:Full Length of Mature Protein

Tag Info:Tag-Free

Mol. Weight:19.4 kDa

Biological_Activity:Fully biologically active when compared to standard. The ED50 as determined by thymidine uptake assay using FGF-receptors transfected BaF3 cells is less than 0.5 ?g/ml, corresponding to a specific activity of ? 2.0 × 103 IU/mg in the presence of 5 µg/ml of rMuKlotho-? and 10 ?g/ml of heparin.

Purity:>96% as determined by SDS-PAGE and HPLC.

Endotoxin:Less than 1.0 EU/µg as determined by LAL method.

Form:Lyophilized powder

Buffer:Lyophilized from a 0.2 µm filtered PBS, pH 7.4

Reconstitution:We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20?/-80?. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Notes:Repeated freezing and thawing is not recommended. Store working aliquots at 4? for up to one week.

Alternative Name/ Alias:FGF-21,

Relevance:Stimulates glucose uptake in differentiated adipocytes via the induction of glucose transporter SLC2A1/GLUT1 expression (but not SLC2A4/GLUT4 expression). Activity requires the presence of KLB. {ECO:0000269|PubMed:15902306, ECO:0000269|PubMed:17623664}.

PubMed ID:10858549; 12975309; 15489334; 15340161; 15902306; 17623664; 18829467; 19117008; 20094046

Function:Stimulates glucose uptake in differentiated adipocytes via the induction of glucose transporter SLC2A1/GLUT1 expression (but not SLC2A4/GLUT4 expression). Activity requires the presence of KLB.

Involvement in disease:

Subcellular Location:Secreted

Protein Families:Heparin-binding growth factors family

Tissue Specificity:

Paythway:MAPKsignalingpathway

HGNC Database Link:https://www.genenames.org/cgi-bin/gene_symbol_report?hgnc_id=HGNC:3678

UniGene Database Link:https://www.ncbi.nlm.nih.gov/UniGene/clust.cgi?ORG=Hs&CID=283015

KEGG Database Link:https://www.genome.jp/dbget-bin/www_bget?hsa:26291

STRING Database Link:https://string-db.org/network/9606.ENSP00000222157

OMIM Database Link:https://www.omim.org/entry/609436609436609436

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