Size:20ug. Other sizes are also available. For further information, please contact us.
Research Areas:Cell Biology
Uniprot ID:Q99961
Gene Names:SH3GL1
Organism:Homo sapiens (Human)
AA Sequence:MSVAGLKKQFYKASQLVSEKVGGAEGTKLDDDFKEMEKKVDVTSKAVTEVLARTIEYLQPNPASRAKLTMLNTVSKIRGQVKNPGYPQSEGLLGECMIRHGKELGGESNFGDALLDAGESMKRLAEVKDSLDIEVKQNFIDPLQNLCEKDLKEIQHHLKKLEGRRLDFDYKKKRQGKIPDEELRQALEKFEESKEVAETSMHNLLETDIEQVSQLSALVDAQLDYHRQAVQILDELAEKLKRRMREASSRPKREYKPKPREPFDLGEPEQSNGGFPCTTAPKIAASSSFRSSDKPIRTPSRSMPPLDQPSCKALYDFEPENDGELGFHEGDVITLTNQIDENWYEGMLDGQSGFFPLSYVEVLVPLPQ
Expression Region:1-368aa
Sequence Info:Full Length
Source:E.coli
Tag Info:N-terminal 10xHis-tagged
MW:45.0 kDa
Alternative Name(s):EEN fusion partner of MLL (Endophilin-2) (Extra eleven-nineteen leukemia fusion gene protein) (EEN) (SH3 domain protein 2B) (SH3 domain-containing GRB2-like protein 1 CNSA1) (SH3D2B)
Relevance:Implicated in endocytosis. May recruit other proteins to membranes with high curvature
Reference:"EEN encodes for a member of a new family of proteins containing an Src homology 3 domain and is the third gene located on chromosome 19p13 that fuses to MLL in human leukemia." So C.W., Caldas C., Liu M.-M., Chen S.-J., Huang Q.-H., Gu L.-J., Sham M.H., Wiedemann L.M., Chan L.C. Proc. Natl. Acad. Sci. U.S.A. 94:2563-2568(1997)
Purity:Greater than 85% as determined by SDS-PAGE.
Form:Liquid or Lyophilized powder
Buffer:If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution:We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20?/-80?. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Storage:The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20?/-80?. The shelf life of lyophilized form is 12 months at -20?/-80?.
Notes:Repeated freezing and thawing is not recommended. Store working aliquots at 4? for up to one week.
Function:Implicated in endocytosis. May recruit other proteins to membranes with high curvature (By similarity).
Involvement in disease:In some cases of acute leukemia, a translocation results in the formation of a KMT2A/MLL1-EEN fusion gene.
Subcellular Location:Cytoplasm, Early endosome membrane, Peripheral membrane protein, Cell projection, podosome
Protein Families:Endophilin family
Tissue Specificity:Ubiquitous. Higher expression in pancreas, placenta, prostate, testis and uterus.
Paythway:Endocytosis
HGNC Database Link:https://www.genenames.org/cgi-bin/gene_symbol_report?hgnc_id=HGNC:10830
UniGene Database Link:https://www.ncbi.nlm.nih.gov/UniGene/clust.cgi?ORG=Hs&CID=490415
KEGG Database Link:https://www.genome.jp/dbget-bin/www_bget?hsa:6455
STRING Database Link:https://string-db.org/network/9606.ENSP00000269886
OMIM Database Link:https://www.omim.org/entry/601768601768601768
Lead Time Guidance:3-7 business days