Size:100ug. Other sizes are also available. Please contact us.
Research Areas:Immunology
Uniprot NO.:O00585
Uniprot Entry Name:CCL21_HUMAN
Gene Names:CCL21,SCYA21,UNQ784/PRO1600
Species:Homo sapiens (Human)
Source:E.Coli
Expression Region:24-134aa
Sequence:SDGGAQDCCL KYSQRKIPAK VVRSYRKQEP SLGCSIPAIL FLPRKRSQAE LCADPKELWV QQLMQHLDKT PSPQKPAQGC RKDRGASKTG KKGKGSKGCK RTERSQTPKG P
Protein Description:Full Length of Mature Protein
Tag Info:Tag-Free
Mol. Weight:12.2 kDa
Biological_Activity:Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human lymphocytes is in a concentration range of 10-100 ng/ml.
Purity:>97% as determined by SDS-PAGE and HPLC.
Endotoxin:Less than 1.0 EU/µg as determined by LAL method.
Form:Lyophilized powder
Buffer:Lyophilized from a 0.2 µm filtered PBS, pH 7.4
Reconstitution:We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20?/-80?. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Notes:Repeated freezing and thawing is not recommended. Store working aliquots at 4? for up to one week.
Alternative Name/ Alias:6Ckine, Secondary lymphoid-tissue chemokine, SLC, Small-inducible cytokine A21,
Relevance:Inhibits hemopoiesis and stimulates chemotaxis. Chemotactic in vitro for thymocytes and activated T-cells, but not for B-cells, macrophages, or neutrophils. Shows preferential activity towards naive T-cells. May play a role in mediating homing of lymphocytes to secondary lymphoid organs. Binds to atypical chemokine receptor ACKR4 and mediates the recruitment of beta-arrestin (ARRB1/2) to ACKR4.
PubMed ID:9235955; 9257816; 9300671; 9419363; 12975309; 15489334; 9507024; 23341447; 22221265
Function:Inhibits hemopoiesis and stimulates chemotaxis. Chemotactic in vitro for thymocytes and activated T-cells, but not for B-cells, macrophages, or neutrophils. Shows preferential activity towards naive T-cells. May play a role in mediating homing of lymphocytes to secondary lymphoid organs. Binds to atypical chemokine receptor ACKR4 and mediates the recruitment of beta-arrestin (ARRB1/2) to ACKR4.
Involvement in disease:
Subcellular Location:Secreted
Protein Families:Intercrine beta (chemokine CC) family
Tissue Specificity:Highly expressed in high endothelial venules of lymph nodes, spleen and appendix. Intermediate levels found in small intestine, thyroid gland and trachea. Low level expression in thymus, bone marrow, liver, and pancreas. Also found in tonsil, fetal heart and fetal spleen.
Paythway:Chemokinesignalingpathway
HGNC Database Link:https://www.genenames.org/cgi-bin/gene_symbol_report?hgnc_id=HGNC:10620
UniGene Database Link:https://www.ncbi.nlm.nih.gov/UniGene/clust.cgi?ORG=Hs&CID=57907
KEGG Database Link:https://www.genome.jp/dbget-bin/www_bget?hsa:6366
STRING Database Link:https://string-db.org/network/9606.ENSP00000259607
OMIM Database Link:https://www.omim.org/entry/602737602737602737