Size:100ug. Other sizes are also available. Please contact us.
Research Areas:Microbiology
Uniprot NO.:P81534
Uniprot Entry Name:D103A_HUMAN
Gene Names:DEFB103A,BD3,DEFB103,DEFB3;,DEFB103B
Species:Homo sapiens (Human)
Source:E.Coli
Expression Region:23-67aa
Sequence:GIINTLQKYY CRVRGGRCAV LSCLPKEEQI GKCSTRGRKC CRRKK
Protein Description:Full Length of Mature Protein
Tag Info:Tag-Free
Mol. Weight:5.2 kDa
Biological_Activity:Fully biologically active when compared to standard. The ED50 as determined by anti-microbial activity against E.coli. is less than 30 ?g/ml, corresponding to a specific activity of ? 33.3 IU/mg.
Purity:>98% as determined by SDS-PAGE and HPLC.
Endotoxin:Less than 1.0 EU/µg as determined by LAL method.
Form:Lyophilized powder
Buffer:Lyophilized from a 0.2 µm filtered 20 mM PB, pH 7.4, 130 mM NaCl
Reconstitution:We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20?/-80?. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Notes:Repeated freezing and thawing is not recommended. Store working aliquots at 4? for up to one week.
Alternative Name/ Alias:Beta-defensin 3, DEFB-3, HBD3, hBD-3, Defensin, beta 103
Relevance:Exhibits antimicrobial activity against Gram-positive bacteria S.aureus and S.pyogenes, Gram-negative bacteria P.aeruginosa and E.coli and the yeast C.albicans. Kills multiresistant S.aureus and vancomycin-resistant E.faecium. No significant hemolytic activity was observed. {ECO:0000269|PubMed:11085990}.
PubMed ID:11085990; 11702237; 11223260; 11741980
Function:Exhibits antimicrobial activity against Gram-positive bacteria S.aureus and S.pyogenes, Gram-negative bacteria P.aeruginosa and E.coli and the yeast C.albicans. Kills multiresistant S.aureus and vancomycin-resistant E.faecium. No significant hemolytic activity was observed.
Involvement in disease:
Subcellular Location:Secreted
Protein Families:Beta-defensin family
Tissue Specificity:Highly expressed in skin and tonsils, and to a lesser extent in trachea, uterus, kidney, thymus, adenoid, pharynx and tongue. Low expression in salivary gland, bone marrow, colon, stomach, polyp and larynx. No expression in small intestine.
Paythway:
HGNC Database Link:https://www.genenames.org/cgi-bin/gene_symbol_report?hgnc_id=HGNC:15967
UniGene Database Link:https://www.ncbi.nlm.nih.gov/UniGene/clust.cgi?ORG=Hs&CID=283082
KEGG Database Link:https://www.genome.jp/dbget-bin/www_bget?hsa:414325
STRING Database Link:https://string-db.org/network/9606.ENSP00000324633
OMIM Database Link:https://www.omim.org/entry/606611606611606611