Recombinant Human 5'-nucleotidase(NT5E) (Active)

Recombinant Human 5'-nucleotidase(NT5E) (Active)

CSB-MP723415HU
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Size:100ug. Other sizes are also available. Please contact us.

Research Areas:Epigenetics and Nuclear Signaling

Uniprot NO.:P21589

Uniprot Entry Name:

Gene Names:NT5E

Species:Homo sapiens (Human)

Source:Mammalian cell

Expression Region:27-549aa

Sequence:WELTILHTNDVHSRLEQTSEDSSKCVNASRCMGGVARLFTKVQQIRRAEPNVLLLDAGDQYQGTIWFTVYKGAEVAHFMNALRYDAMALGNHEFDNGVEGLIEPLLKEAKFPILSANIKAKGPLASQISGLYLPYKVLPVGDEVVGIVGYTSKETPFLSNPGTNLVFEDEITALQPEVDKLKTLNVNKIIALGHSGFEMDKLIAQKVRGVDVVVGGHSNTFLYTGNPPSKEVPAGKYPFIVTSDDGRKVPVVQAYAFGKYLGYLKIEFDERGNVISSHGNPILLNSSIPEDPSIKADINKWRIKLDNYSTQELGKTIVYLDGSSQSCRFRECNMGNLICDAMINNNLRHTDEMFWNHVSMCILNGGGIRSPIDERNNGTITWENLAAVLPFGGTFDLVQLKGSTLKKAFEHSVHRYGQSTGEFLQVGGIHVVYDLSRKPGDRVVKLDVLCTKCRVPSYDPLKMDEVYKVILPNFLANGGDGFQMIKDELLRHDSGDQDINVVSTYISKMKVIYPAVEGRIKFS

Protein Description:Full Length of Mature Protein

Tag Info:C-terminal 6xHis-tagged

Mol. Weight:60.2

Biological_Activity:Measured by its binding ability in a functional ELISA. Immobilized CD73 at 2 ?g/ml can bind Anti- CD73 Rabbit Monoclonal Antibody?CSB-RA978310A0HU?, the EC50 is 3.212-4.525 ng/ml.

Purity:Greater than 95% as determined by SDS-PAGE.

Endotoxin:Less than 1.0 EU/ug as determined by LAL method.

Form:Lyophilized powder

Buffer:Lyophilized from a 0.2 ?m filtered 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0

Reconstitution:We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20?/-80?. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Notes:Repeated freezing and thawing is not recommended. Store working aliquots at 4? for up to one week.

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Paythway:

HGNC Database Link:

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STRING Database Link:

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