Size:100ug. Other sizes are also available. For further information, please contact us.
Research Areas:Others
Uniprot ID:B7IR00
Gene Names:uvsE
Organism:Bacillus cereus (strain G9842)
AA Sequence:MIMRFGYVSHAMALWDCSPAKTMTFTSFKKLSKQEREDKLYHVIRQNLEHTIRILHYNIAHEIPLYRLSSSIVPLATHPEVEFDYIGVFTPLWRKIGALIKEHNLRISFHPNQFTLFTSDKPHITTNAITDMTYHYKILDAIGIADSSYINIHVGGAYGNKEKAIERFHENIKKLPAHIKKQMTLENDDKTYTTSETLSICQKENIPFVFDYHHHMANLCEQPLEELLPAIFETWSHTNISPKVHISSPRSEKEFRAHAEYIDLEFIKPFLHVAKKNNHNFDIMIESKQKDLALFRLIDELSAIRGIKRISGAMLQW
Expression Region:1-317aa
Sequence Info:Full Length
Source:E.coli
Tag Info:N-terminal 10xHis-tagged and C-terminal Myc-tagged
MW:44.0 kDa
Alternative Name(s):uvsE; BCG9842_B5482; UV DNA damage endonuclease; UV-endonuclease; UVED; EC 3.-.-.-
Relevance:Component in a DNA repair pathway. Removal of UV LIGHT damaged nucleotides. Recognizes pyrimidine dimers and cleave a phosphodiester bond immediately 5' to the lesion
Reference:
Purity:Greater than 85% as determined by SDS-PAGE.
Form:Liquid or Lyophilized powder
Buffer:If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution:We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20?/-80?. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Storage:The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20?/-80?. The shelf life of lyophilized form is 12 months at -20?/-80?.
Notes:Repeated freezing and thawing is not recommended. Store working aliquots at 4? for up to one week.
Function:Component in a DNA repair pathway. Removal of UV LIGHT damaged nucleotides. Recognizes pyrimidine dimers and cleave a phosphodiester bond immediately 5' to the lesion (By similarity).
Involvement in disease:
Subcellular Location:
Protein Families:Uve1/UvsE family
Tissue Specificity:
Paythway:
HGNC Database Link:
UniGene Database Link:
KEGG Database Link:https://www.genome.jp/dbget-bin/www_bget?bcg:BCG9842_B5482
STRING Database Link:
OMIM Database Link:
Lead Time Guidance:13-23 business days