Recombinant Arabidopsis thaliana UDP-glycosyltransferase 78D1(UGT78D1)

Recombinant Arabidopsis thaliana UDP-glycosyltransferase 78D1(UGT78D1)

CSB-EP871008DOA
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Size:20ug. Other sizes are also available. For further information, please contact us.

Research Areas:Others

Uniprot ID:Q9S9P6

Gene Names:UGT78D1

Organism:Arabidopsis thaliana (Mouse-ear cress)

AA Sequence:MTKFSEPIRDSHVAVLAFFPVGAHAGPLLAVTRRLAAASPSTIFSFFNTARSNASLFSSDHPENIKVHDVSDGVPEGTMLGNPLEMVELFLEAAPRIFRSEIAAAEIEVGKKVTCMLTDAFFWFAADIAAELNATWVAFWAGGANSLCAHLYTDLIRETIGLKDVSMEETLGFIPGMENYRVKDIPEEVVFEDLDSVFPKALYQMSLALPRASAVFISSFEELEPTLNYNLRSKLKRFLNIAPLTLLSSTSEKEMRDPHGCFAWMGKRSAASVAYISFGTVMEPPPEELVAIAQGLESSKVPFVWSLKEKNMVHLPKGFLDRTREQGIVVPWAPQVELLKHEAMGVNVTHCGWNSVLESVSAGVPMIGRPILADNRLNGRAVEVVWKVGVMMDNGVFTKEGFEKCLNDVFVHDDGKTMKANAKKLKEKLQEDFSMKGSSLENFKILLDEIVKV

Expression Region:1-453aa

Sequence Info:Full Length

Source:E.coli

Tag Info:N-terminal 10xHis-tagged

MW:55.6 kDa

Alternative Name(s):Flavonol-3-O-glucoside L-rhamnosyltransferase UDP-rhamnose:flavonol 3-O-glucoside rhamnosyltransferase

Relevance:Acts as a UDP-rhamnose:flavonol-3-O-glucoside rhamnosyltransferase. Keampferol and quercitin are used as substrates. Possesses low quercetin 3-O-glucosyltransferase activity in vitro.

Reference:"Phylogenetic analysis of the UDP-glycosyltransferase multigene family of Arabidopsis thaliana." Li Y., Baldauf S., Lim E.K., Bowles D.J. J. Biol. Chem. 276:4338-4343(2001)

Purity:Greater than 85% as determined by SDS-PAGE.

Form:Liquid or Lyophilized powder

Buffer:If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Reconstitution:We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20?/-80?. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Storage:The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20?/-80?. The shelf life of lyophilized form is 12 months at -20?/-80?.

Notes:Repeated freezing and thawing is not recommended. Store working aliquots at 4? for up to one week.

Function:Acts as a UDP-rhamnose

Involvement in disease:

Subcellular Location:

Protein Families:UDP-glycosyltransferase family

Tissue Specificity:Expressed in leaves, flowers, siliques, and stems.

Paythway:

HGNC Database Link:

UniGene Database Link:https://www.ncbi.nlm.nih.gov/UniGene/clust.cgi?ORG=At&CID=21995

KEGG Database Link:https://www.genome.jp/dbget-bin/www_bget?ath:AT1G30530

STRING Database Link:https://string-db.org/network/3702.AT1G30530.1

OMIM Database Link:

Lead Time Guidance:3-7 business days

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