Size:100ul
Host:Rabbit
Clonality:Polyclonal
Isotype:IgG
GeneName:AKT1S1
GeneID:84335
Uniprot:Q96B36
Specificity:Phospho-PRAS40 (Thr246) Antibody detects endogenous levels of PRAS40 only when phosphorylated at Thr246.
Immunogen:A synthesized peptide derived from human PRAS40 around the phosphorylation site of Thr246.
Storage:Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.
Reactivity:Human,Mouse,Rat
Prediction:Pig,Zebrafish,Bovine,Horse,Sheep,Dog,Xenopus
Application:WB,IHC
Dilution:WB 1:500-1:2000, IHC 1:50-1:200
Conjugate:Unconjugated
RRID:AB_2845401
An Introduction to Blocking Peptides:A superior strategy for validation of antibody binding is the use of blocking peptides. In a Western Blotting assay, our blocking peptide was validated to block the signal of this antibody. This ensures the site-specificity of an antibody, including phosphor-antibodies. In the case of the phosphor- antibodies, the antibodies can and will only recognize the phosphorylated target protein at the corresponding site, not the non-phosphorylated target protein or target protein phosphorylated at a different site.
Cat# Blocking Peptide:AF2387-BP
Des# Blocking Peptide:Phospho-PRAS40 (Thr246) Blocking peptide
Blocking Peptide Price:$549 CAD