This product is a 74.4 kDa purified recombinant protein inducibly expressed in E. coli carrying the φ29 DNA polymerase gene. With high sensitivity and great synthesis ability, it can be used for whole genome amplification from a single cell. It possesses 3'→5' proofreading exonuclease activity to ensure high fidelity of resulting DNA. Its properties of multiple strand displacement and processive synthesis allow >10 kb lengths of amplification products from genome or plasmid DNA, and enable rolling-circle replication of circular DNA.
Highlights
· Isothermal amplification;
· High fidelity; high efficiency; high sensitivity; high yield;
· Random primers or specific N9 primers can be used.
Application
Sequencing, SNP genotyping, STR/ microsatellite analysis, virus detection, miRNA detection, etc.
Storage
at -20°C for two years
Shipping
Dry ice (-70 ℃)
| Component | LP101-01 | LP101-02 |
| Phi29 DNA Polymerase | 250 units | 5×250 units |
| 10×Phi29 DNA Polymerase Buffer | 50 μl | 250 μl |
| Phi29 Random Primers | 125 μl | 625 μl |
| 10 mM dNTPs | 25 μl | 125 μl |
| 10×DNA Loading Buffer | 1 ml | 1 ml |