This product is a ready-to-use qPCR cocktail. It contains a PerfectStart® Taq DNA polymerase, optimized dual-cation buffer, InFluor™ Green*, dNTPs, PCR enhancer, PCR stabilizer and optimized Universal Passive Reference Dyes in an optimized formulation such that this single reagent works for all major real-time (q)PCR systems, including all Bio-rads and Applied Bio (ThermoFisher)'s models. This reagent contains a Universal Passive Reference Dye, and is suitable for replacing those known as No ROX, Low ROX or High ROX master mixes, recommended depending on which real-time PCR systems you have in you lab.
PerfectStart® Taq DNA polymerase is a hot-start Taq DNA polymerase containing Taq DNA polymerase and three kinds of monoclonal antibodies, effectively blocking DNA polymerase activity and preventing non-specific amplification at low temperature. qPCR SuperMix is provided at 2× concentration and can be used at 1× concentration by adding template, primers, and nuclease-free water.
*About InFluor™ Green: a nucleic acid (DNA and RNA) intercalating dye, which is fully compatible will all major real-time PCR (qPCR) systems, specifically, InFluor™ Green fluorescence can be read in the green channel of the real-time PCR thermal cyclers for quantitative PCR analyses. The InFluor™ does not inhibit PCR amplification. It supports melt curve analyses.
Highlights
• PerfectStart® Taq DNA Polymerase enables high specificity, high sensitivity, and high amplification efficiency for your quantitative PCR.
• Dual-cation buffer enhances specificity and minimizes the tendency of primer-dimer formation.
• Blocking of polymerase activity by 3 antibodies; high specificity, sensitivity and amplification efficiency; applicable to a wide range of species.
• Universal Passive Reference Dye for different instruments, eliminating the need use different levels of ROX reference dye, depending on if the real-time PCR system requires No ROX, Low ROX or High ROX. This means that you do not need to worry about buying the wrong Green qPCR master mix. This also means that if you have a Bio-Rad machine and an ABI system, you can just stock this single reagent.
Performance comparison
According to our manufacturer, PerfectStart® Universal AQ602 was found to provide better standard curve when compared with wth a leading brand (V). See below. In addition, our customers have also shared with us data to suggest superior performance of this product when compared to another brand name.
