Product Details
This kit takes advantage of proprietary assembly mix and homologous recombination. This kit can achieve directional cloning of PCR fragments that share 15-25 bp overlapping sequences into any linearized vector.
Features
- Fast: 5~15 minutes
- Simple: No restriction enzyme digestions.
- High efficiency: > 95% cloning efficiency.
- Seamless: No extra sequences introduced.
Applications
Single or multi fragments cloning and assembly.
Storage
-18℃ or below for two years
Shipping
Ice/gel packs (4℃)
Product Composition
|
Component |
CU101-01 (10rxns) |
CU101-02 (20rxns) |
CU101-03 (60 rxns) |
|
2×Assembly Mix |
50 μl |
100 μl |
3×100 μl |
|
Linearized pUC19 Control Vector (10 ng/μl) |
3 μl |
6 μl |
18 μl |
|
Control Insert (1 kb，20 ng/μl） |
3 μl |
6 μl |
18 μl |
Cloning competent cells can be ordered with this cloning kit. Please ask us for help if you need to add the competent cells to your order of this cloning kit.
SOC Medium and SOB Medium may be purchased with this cloning kit.
References:
| Literature | Journal | IF | Author | Date | Link |
|---|---|---|---|---|---|
| Switch of metabolic status: redirecting metabolic flux for acetoin production from glycerol by activating a silent glycerol catabolism pathway | METABOLIC ENGINEERING | 8.142 | Wang Y, et al. State Key Laboratory of Microbial M | 2017 Jan | The original link |
| A novel nematode effector suppresses plant immunity by activating host reactive oxygen species-scavenging system | new phytologist | 7.21 | Borong Lin, et al. | 2015 Oct | The original link |
| Phosphorylation-mediated Regulatory Networks in Mycelia of Pyricularia oryzae Revealed by Phosphoproteomic Analyses | MOLECULAR & CELLULAR PROTEOMICS | 6.5399 | Ruijin Wang,et al. | 2017Jul | The original link |
| The maize transcription factor EREB58 mediates the jasmonate-induced production of sesquiterpene volatiles. | Plant journal | 5.4679 | Li S, et al. Biotechnology Research Institute, Chi | 2015 Oct | The original link |
| Characterization of Pseudooxynicotine Amine Oxidase of Pseudomonas putida S16 that Is Crucial for Nicotine Degradation. | Scientific Reports | 5.2278 | Hu H, et al. State Key Laboratory of Microbial Met | 2015 Dec | The original link |