This kit takes advantage of proprietary recombinases and homologous recombination to achieve directional recombination of PCR inserts with 15-25bp overlapping ends into any linearized vector, enabling efficient and seamless assembly of 1-5 fragments.
Features
• Fast: 5~15 minutes.
• Simple: No restriction enzyme digestions.
• High efficiency: > 95% cloning efficiency.
• Seamless: No extra sequences introduced.
Applications
Single or multi fragments cloning and assembly.
Storage
at -20℃ for two years.
Shipping
Ice/gel packs (4℃)
Product Composition
|
Component |
CU201-02 |
CU201-03 |
|
2×Basic Assembly Mix |
100 μl |
3×100 μl |
|
Linearized pUC19 Control Vector (10 ng/μl) |
6 μl |
18 μl |
|
Control Insert (1 kb，20 ng/μl） |
6 μl |
18 μl |
References
1 Zou Z P, Du Y, Fang T T, et al. Biomarker-responsive engineered probiotic diagnoses, records, and ameliorates inflammatory bowel disease in mice[J]. Cell Host & Microbe, 2023.(IF 31.31)
2 Wan W, Dong H, Lai D H, et al. The Toxoplasma micropore mediates endocytosis for selective nutrient salvage from host cell compartments[J]. Nature communications, 2023.(IF 17.69)
3 You L, Omollo E O, Yu C, et al. Structural basis for intrinsic transcription termination[J]. Nature, 2023.(IF 69.50)
4 Zhang H W, Huang K, Gu Z X, et al. A cryo-EM structure of KTF1-bound polymerase V transcription elongation complex[J]. Nature Communications, 2023.(IF 17.69)
5 Huang J, Yang L, Yang L, et al. Stigma receptors control intraspecies and interspecies barriers in Brassicaceae[J]. Nature, 2023(IF 64.80)
6 Tang J, Zhang G, Li F, et al. Two-Dimensional Protein Nanoarray as a Carrier of Sensing Elements for Gold-Based Immunosensing Systems[J]. Analytical Chemistry, 2022.(IF 8.00)
7 Wu M, Xu G, Han C, et al. lncRNA SLERT controls phase separation of FC/DFCs to facilitate Pol I transcription[J]. Science, 2021.(IF 63.71)
8 Chen Q, Hu T, Li X, et al. Phosphorylation of SWEET sucrose transporters regulates plant root: shoot ratio under drought[J]. Nature plants, 2022.(IF 15.79)
9 Liu R, Yang J, Yao J, et al. Optogenetic control of RNA function and metabolism using engineered light-switchable RNA-binding proteins[J]. Nature biotechnology, 2022.(IF 54.90)
10 Wu M, Xu G, Han C, et al. lncRNA SLERT controls phase separation of FC/DFCs to facilitate Pol I transcription[J]. Science, 2021.(IF 63.71)
11 Zeng Y H, Cai Z H, Zhu J M, et al. Two hierarchical LuxR-LuxI type quorum sensing systems in Novosphingobium activate microcystin degradation through transcriptional regulation of the mlr pathway[J]. Water Research, 2020.(IF 9.13)