Size:100ul
Host:Rabbit
Clonality:Polyclonal
Isotype:IgG
GeneName:HIST3H3
GeneID:8290
Uniprot:Q16695
Specificity:Mono-Methyl-Histone H3 (Lys14)/H3K14me1 Antibody detects endogenous levels of Mono-Methyl-Histone H3 only when methylated at Lys14.
Immunogen:A synthetic methylated peptide derived from human Mono-Methyl-Histone H3 around the methylation site of Lys14.
Storage:Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.
Reactivity:Human,Mouse,Rat
Prediction:
Application:WB,IF/ICC,ELISA(peptide)
Dilution:WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:20-1:50, ELISA(peptide) 1:20000-1:40000
Conjugate:Unconjugated
RRID:AB_2839190
An Introduction to Blocking Peptides:A superior strategy for validation of antibody binding is the use of blocking peptides. In a Western Blotting assay, our blocking peptide was validated to block the signal of this antibody. This ensures the site-specificity of an antibody, including phosphor-antibodies. In the case of the phosphor- antibodies, the antibodies can and will only recognize the phosphorylated target protein at the corresponding site, not the non-phosphorylated target protein or target protein phosphorylated at a different site.
Cat# Blocking Peptide:DF7251-BP
Des# Blocking Peptide:Mono-Methyl-Histone H3 (Lys14)/H3K14me1 Blocking peptide
Blocking Peptide Price:$549 CAD