The Low Range ladder is ideal for determining the size of double-stranded DNA from 25 to 700 base pairs. The ladder consists of 10 linear double-stranded fragments .The 100bp and 300bp fragment are present at increased intensity to allow easy identification. All fragments are precisely quantified and mixed during the production. For 5 ul loading, all fragments except 100bp and 200bp are 40ng. The 100bp and 200bp fragment is 100ng. This ladder is pre-mixed with loading dye and is ready to use.
Recommended Loading
2-5 µl/Lane
Concentration
Typical Bands 100 ng/
Other Bands 40 ng/5µl
Advised Electrophoresis Condition
8 cm, 3% Agarose Gel, 0.5×TBE, 5 V/cm, 1 h
Contents(bp)
25、50 、75、100、150、200、300、400、500、700
Mix Concentration
104 ng/µl
Storage
Stable for 3 months at RT, for long-time storage, please store at -20℃.