Introduction
The HiPure Universal DNA Kit—a reliable and efficient solution for the quick extraction of total DNA from various clinical samples, such as tissue, cells, blood, saliva, swabs, blood spots, and semen. The extracted DNA is ready for immediate use in PCR, qPCR, Southern Blot analysis, and viral DNA testing.
Specifications
| Features | Specifications |
| Main Functions | Isolation total DNA from tissue / blood / body fluid / swab /dry blood spots |
| Applications | PCR, qPCR, southern bolt and virus detection, etc |
| Purification method | Mini spin column |
| Purification technology | Silica technology |
| Process method | Manual (centrifugation or vacuum) |
| Sample type | Tissue, cell, blood, saliva, swab, blood spot,semen and other clinical samples |
| Sample amount | Solid tissue: 1-10mg; Anticoagulant blood: 200μl |
| Elution volume | ≥20μl |
| Time per run | 30 - 60 minutes |
| Liquid carrying volume per column | 800µl |
| Binding yield of column | 100µg |
Principle
This kit utilizes silica column-based purification technology. The process starts with the lysis and proteolytic digestion of the sample, releasing DNA into the lysate. The lysate is then transferred to an adsorption column where nucleic acids bind to the membrane. Proteins and other impurities are washed away, and the purified nucleic acid is eluted with a low-salt buffer (10 mM Tris, pH 9.0, 0.5 mM EDTA).
Advantages
- High quality DNA - meet a variety of downstream applications, including PCR, qPCR, enzyme digestion, hybridization, etc.
- Fast - without separation of leukocytes, organic extraction or ethanol precipitation
- Simple - all nucleic acids can be obtained by direct digestion
- Wide applicability- handle a variety of liquid samples
Kit Contents
| Contents | D301802 | D301803 |
| Purification Times | 50 | 250 |
| HiPure DNA Mini Columns I | 50 | 250 |
| 2ml Collection Tubes | 100 | 500 |
| Buffer ATL | 30 ml | 150 ml |
| Buffer AL | 30 ml | 150 ml |
| Buffer GW1 | 22 ml | 88 ml |
| Buffer GW2 | 12 ml | 50 ml |
| Proteinase K | 24 mg | 120 mg |
| Protease Dissolve Buffer | 1.8 ml | 10 ml |
| Buffer AE | 15 ml | 60 ml |
Storage and Stability
Proteinase K should be stored at 2-8°C upon arrival. However, short-term storage (up to 12 weeks) at room temperature (15-25°C) does not affect their performance. The remaining kit components can be stored at room temperature (15-25°C) and are stable for at least 18 months under these conditions. The entire kit can be stored at 2-8°C, but in this case buffers should be redissolved before use. Make sure that all buffers are at room temperature when used.
