FlyCut® XhoI is expressed and purified from E.coli that carries the recombinant XhoI gene. The molecular weight is 27.9 kDa, with the recognition site at C^TCGAG. The reaction is conducted for 5-15 minutes at 37℃, and heat-inactivated at 65℃for 20 minutes. This enzyme is not sensitive to dam or dcm methylation, but sensitive to mammalian CpG methylation.
Recognition Site
5’...C↓TCGAG...3’
3’...GAGCT↑C...5’
Applications
Genomic DNA, plasmid DNA, PCR product
Storage
at -20°C for two years
Shipping
Dry ice (-70 ℃)
| Component | JX201-01 | JX201-02 |
| FlyCut® XhoI | 2,500 units | 2×2,500 units |
| 10×FlyCut® Buffer | 1 ml | 2×1 ml |
| 10×DNA Loading Buffer | 1 ml | 1 ml |