FlyCut® NotI is expressed and purified from E.coli that carries the recombinant NotI gene. The molecular weight is 43.3 kDa, with the recognition site at GC^GGCCGC. The reaction is conducted for 5-15 minutes at 37℃, and heat-inactivated at 65℃ for 20 minutes. This enzyme is not sensitive to dam or dcm methylation, but sensitive to mammalian CpG methylation.
Recognition Site
5’...GC↓GGCCGC...3’
3’...CGCCGG↑CG...5’
Applications
Genomic DNA, plasmid DNA, PCR product
Storage
at -20°C for two years
Shipping
Dry ice (-70 ℃)
| Component | JN401-01 | JN401-02 |
| FlyCut® NotI | 250 units | 2×250 units |
| 10×FlyCut® Buffer | 250 μl | 250 μl |
| 10×DNA Loading Buffer | 1 ml | 1 ml |