FlyCut® NheI is expressed and purified from E.coli that carries the recombinant NheI gene. The molecular weight is 39.1 kDa, with the recognition site at G^CTAGC. The reaction is conducted for 5-15 minutes at 37℃, and heat-inactivated at 80℃ for 20 minutes. This enzyme is not sensitive to dam or dcm methylation, but sensitive to mammalian CpG methylation.
Recognition Site
5’...G↓CTAGC...3’
3’...CGATC↑G...5’
Applications
Genomic DNA, plasmid DNA, PCR product
Storage
at -20°C for two years
Shipping
Dry ice (-70 ℃)
| Component | JN301-01 | JN301-02 |
| FlyCut® NheI | 500 units | 2×500 units |
| 10×FlyCut® Buffer | 250 μl | 250 μl |
| 10×DNA Loading Buffer | 1 ml | 1 ml |