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Product Overview: The Equalbit 1 × dsDNA HS Assay Kit is a highly sensitive and accurate fluorescence-based detection kit designed for the quantification of double-stranded DNA (dsDNA). This kit includes a pre-mixed working solution with fluorescent dye and dsDNA standards, ensuring a simple and efficient workflow. It offers excellent linearity within the range of 0.2 - 100 ng dsDNA, allowing for precise quantification of dsDNA concentrations from 10 pg/μl to 100 ng/μl. The kit is also highly tolerant to common contaminants such as RNA, salts, free nucleotides, proteins, solvents, and detergents, making it a robust choice for various applications.
Key Features:
- Simple Operation: The ready-to-use pre-mixed working solution simplifies the assay process by eliminating the need for additional preparation steps.
- High Sensitivity and Precision: Accurately quantifies dsDNA in the range of 0.2 - 100 ng, with a detection limit as low as 10 pg/μl.
- High Specificity: Specifically detects dsDNA while tolerating common contaminants, ensuring reliable results.
- Rapid Dye Binding: Achieves saturation within 2 minutes, allowing for quick and efficient assays.
- High Stability: The production process of standards is strictly controlled to ensure lot-to-lot consistency and stability.
Components:
- Equalbit 1 × dsDNA HS Working Solution
- Equalbit 1 × dsDNA HS Standard #1 (0 ng/μl in TE buffer)
- Equalbit 1 × dsDNA HS Standard #2 (10 ng/μl in TE buffer)
Storage Instructions:
- Store the kit at 2 ~ 8°C and protect it from light.
- For long-term storage, store Equalbit 1 × dsDNA HS Standard #2 at -30 ~ -15°C.
- Adjust the shipping method according to different destinations to maintain product integrity.
Applications:
- Suitable for the detection of dsDNA concentrations ranging from 10 pg/μl to 100 ng/μl.
- Ideal for use with the Qubit Fluorometer for simple and accurate dsDNA quantification.
Notes:
- To avoid contamination, transfer a sufficient amount of the working solution to a centrifuge tube before use.
- Mix standards and samples thoroughly by inversion before use to ensure accurate results.
- Use a calibrated pipette to ensure the accuracy of quantitative results.
- Perform the quantitative assay at room temperature and avoid prolonged exposure to light during the experiment.
- Complete the detection within 3 hours of adding samples to prevent fluorescence quenching.
Experiment Process:
- Equilibrate all components to room temperature before use.
- Prepare 0.5 ml PCR tubes for all samples and standards.
- Label the lid of each standard and sample tube correctly.
- Prepare the standard solution by adding 190 µl of the working solution to standard PCR tubes, then add 10 µl of Standard #1 and Standard #2 to the corresponding tubes. Gently vortex to avoid bubbles.
- Prepare the sample solution by adding 180 - 199 µl of the working solution to sample PCR tubes, then add 1 - 20 µl of dsDNA samples to achieve a final volume of 200 µl. Gently vortex to avoid bubbles.
- Incubate all PCR tubes at room temperature for 2 minutes, protecting them from light.
- Follow the Qubit Fluorometer instructions to assay the dsDNA concentration using the dsDNA High Sensitivity Assay program.