The kit is designed for the isolation of RNA from various fresh and dried plant samples, including plant samples rich in polysaccharides and polyphenols. The kit is based on unique extraction system and membrane technology to specifically remove secondary metabolites such as polysaccharides, polyphenols, and lipids in samples, without the need for toxic reagents such as phenol and chloroform. Purified RNA with high quality and good stability can be used for RT-PCR, qRT-PCR, Northern blot, RACE, library construction, etc.
Features
- Wide range of applications: applicable to various plant tissues, especially those rich in polysaccharides, polyphenols or starch
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DNase I removes contaminating DNA from RNA samples.
- Ensures RNA purity for accurate downstream applications like qPCR and RNA sequencing.
- Fast operation: high-quality genomic RNA can be extracted in less than 30 minutes
- Safe and low toxicity: no phenol and chloroform
- High purity: the unique technology can efficiently remove impurities such as pigments, polyphenols and polysaccharides in the sample
See protocol details in manual:
When dealing with woody or uncommon samples, The HiPure Plant RNA Plus Kit (R415002) and EasyPure® Universal Plant Total RNA Kit (ER302) are recommended first. R415002 contains two polysaccharide/polyphenol lysis buffer, which are amongst the most universal reagents. While ER302 contains an DNase I solution, as well as a strong polysaccharide/polyphenol lysis buffer.
HiPure Plant RNA Mini (R415102) is recommended for handling common economic crop samples for the first time. Strong lysis solution can be used to process easy-extraction samples. The amount of corn or rice leaves samples can reach up to 300mg.
| Component |
ER302-01 (50 rxns) |
ER302-02 (200 rxns) |
| Lysis Buffer 52 (LB52) | 40 ml | 160 ml |
| Precipitation Buffer 52 (PB52） | 10 ml | 40 ml |
| DNase I (3 units/μl) | 1500 U | 6000 U |
| DNase I Reaction Buffer | 4×1 ml | 15 ml |
| Clean Buffer 52 (CB52 ) | 20 ml | 80 ml |
| Wash Buffer 52 (WB52) | 11 ml | 2×22 ml |
| RNase-free Water | 10 ml | 20 ml |
| Filtration Columns with Collection Tubes | 50 | 200 |
| RNA Spin Columns with Collection Tubes Ⅱ | 50 | 200 |
Storage
at room temperature (15-25°C); DNase I at -20°C
Shipping
Ice/Gel Packs (4°C)