Product Details
EasyPure® Blood Genomic DNA Kit provides a simple and convenient way to isolate high quality genomic DNA from 5-250 μl of fresh or frozen blood. Whole blood is incubated with binding/lysis buffer to release DNA. DNA is bound to silica-based column. The isolated DNA is suitable for PCR, restriction enzyme digestion and Southern blot.
Features
• Simple and fast, red cell lysis buffer is no longer needed.
• Complete removal of contaminants and inhibitors.
• DNA yield up to 40 μg.
• Column based purification, no organic extraction or ethanol precipitation.
• Suitable for EDTA, sodium citrate and heparin-anticoagulated fresh or frozen blood in a volume of 5 to 250 μl.
See manual for more details on EE121:
Storage
At room temperature (15-25°C) in a dry place for one year
Shipping
At room temperature
Product Composition
| Component | EE121-01 (50 rxns) | EE121-02 (200 rxns) |
| Binding Buffer 3 (BB3) | 25 ml | 110 ml |
| Clean Buffer 3 (CB3) | 6 ml | 24 ml |
| Wash Buffer (WB3) | 11 ml | 2x22 ml |
| Elution Buffer (EB) | 20 ml | 80 ml |
| RNase A (20 mg/ml) | 500 ul | 2x1 ml |
| Proteinase K (20 mg.ml) | 1 ml | 4x1 ml |
| Genomic Spin Columns with Collection Tubes | 50 each | 200 each |
Procedure
| Component | EE121-01 (50 rxns) | EE121-02 (200 rxns) |
| Clean Buffer 3 (CB3) | 24 ml | 96 ml |
| Wash Buffer 3 (WB3) | 44 ml | 2x88 ml |
References
1 Zhang D Y, Zhang X X, Li F D, et al. Whole-genome resequencing reveals molecular imprints of anthropogenic and natural selection in wild and domesticated sheep[J]. Zoological Research, 2022.(IF 6.97)
2 Zhang D, Zhang X, Li F, et al. Whole-genome resequencing identified candidate genes associated with the number of ribs in Hu sheep[J]. Genomics, 2021.(IF 6.20)