FlyCut® KpnI is expressed and purified from E.coli that carries the recombinant KpnI gene. The molecular weight is 25.9 kDa, with the recognition site at GGTAC^C. The reaction is conducted for 5-15 minutes at 37℃, and heat-inactivated at 80℃ for 20 minutes. This enzyme is not sensitive to dam, dcm or mammalian CpG methylation.
Recognition Site
5’...GGTAC↓C...3’
3’...C↑CATGG...5’
Applications
Genomic DNA, plasmid DNA, PCR product
Storage
at -20°C for two years
Shipping
Dry ice (-70 ℃)
| Component | JK101-01 | JK101-02 |
| FlyCut® KpnI | 2,000 units | 2×2,000 units |
| 10×FlyCut® Buffer | 500 μl | 1 ml |
| 10×DNA Loading Buffer | 1 ml | 1 ml |