The kit contains Phanta Blood Super-Fidelity DNA Polymerase, which is engineered on the basis of Phanta Super-Fidelity DNA Polymerase. The enzyme is highly resistant to PCR inhibitors in whole blood samples, and can amplify whole blood concentrations up to 40%. Coupled with carefully optimized 2 × Phanta Blood Buffer V2, Blood Direct PCR Kit V2 can efficiently amplify genome fragments up to 10 kb from whole blood samples. Phanta Blood Super-Fidelity DNA Polymerase amplification products are blunt-ended, suitable for ClonExpress® rapid cloning kit (C112/C113). The kit is equipped with a Positive control primer mix compatible with mammals and many other vertebrates, which can be used for positive control reactions.
Based on the modified Phanta® Blood Super-Fidelity DNA Polymerase, it has extremely strong amplification efficiency and PCR inhibitor tolerance;
Suitable for various blood samples, compatible with conventional anticoagulants;
Can tolerate up to 40% of whole blood template;
Can efficiently amplify genome fragments up to 7.5 kb from whole blood
Store at -20°C