Product Details
TransTaq® High Fidelity (HiFi) PCR SuperMix I or II is a ready-to-use mixture of TransTaq® High Fidelity (HiFi) DNA polymerase, dNTPs and optimized buffer. TransTaq® High Fidelity (HiFi) PCR SuperMix I is optimized for the amplification of genomic DNA and PCR SuperMix II is optimized for the amplification of λDNA, cDNA or plasmid DNA. The SuperMix is provided at 2× concentration and can be used at 1× concentration by adding template, primers and H2O.
Features
• TransTaq® High Fidelity (HiFi) PCR SuperMix offers 18-fold fidelity as compared to EasyTaq® DNA Polymerase.
• Extension rate is about 1-2 kb/min.
• Template-independent "A" can be generated at the 3' end of the PCR product. PCR products can be directly cloned into pEASY®-T vectors.
• Amplification of genomic DNA fragment up to 15 kb.
Applications
• Complex templates
• GC/AT-rich templates
• Long PCR
• High yield PCR
Storage
at -20 ℃ for two years
Shipping
Dry ice (-70 ℃)
Product Composition
| Component | AS131-01/21 | AS131-02/22 |
| 2×TransTaq® HiFi PCR SuperMix (Mix I)/(Mix II) | 1 ml | 5×1 ml |
| Nuclease-free Water | 1 ml | 5 ml |
References
1 Guo C J, Ma X K, Xing Y H, et al. Distinct processing of lncRNAs contributes to non-conserved functions in stem cells[J]. Cell, 2020.(IF 36.21)