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The unique extension factor, specificity-promoting factors and plateau-inhibiting factor in Phanta Max greatly improve its long-fragment amplification ability, specificity and yield. Phanta Max is capable of amplifying long fragments such as 40 kb λ DNA, 20 kb genomic DNA and 10 kb cDNA. The amplification error rate of Phanta Max is 53-fold lower than that of Taq and 6-fold lower than that of Pfu. In addition,
Phanta Max has a good resistance to PCR inhibitors and can be used for direct PCR amplifications with bacteria, fungi, plants, animal tissues, and even whole blood samples. Phanta Max contains two monoclonal antibodies inhibiting the 5´→3´ polymerase activity and 3´→5´ exonuclease activity at room temperature. Super fidelity and supreme amplification efficiency make Phanta Max the best choice for high fidelity PCR.
2× Phanta Max Master Mix contains Phanta Max Super-Fidelity DNA Polymerase, dNTP, and an optimized buffer system. The amplification can start only with the addition of primer and template, thereby easing PCR setup and improving reproducibility. Protective agents in the 2× Phanta Max Master Mix enable the resistance to repeated freeze-thaw cycles. Amplification will generate blunt-ended products, which are compatible with ClonExpress II One Step Cloning Kit Series (Vazyme, Cat.No. #C112, #C113).