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Description |
TransNGS™ rRNA Depletion Kit (Human/Mouse/Rat) depletes ribosomal RNA from human/mouse/rat total RNA by RNase H digestion, while retains messenger RNA and non-coding RNA. The depleted ribosomal RNA contains both cytoplasmic ribosomal RNA (5S rRNA, 5.8S rRNA, 18S rRNA and 28S rRNA) and mitochodrial ribosomal RNA (12S rRNA and 16S rRNA). The resulting rRNA-depleted RNA is suitable for RNA-Seq, random-primed cDNA synthesis, or other downstream RNA analysis. |
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Features: |
• Remove up to 99% ribosomal RNA from human/mouse/rat total RNA. • Control qPCR Primer Sets are provided to monitor the depletion efficiency of ribosomal RNA and the retention rate of non-ribosomal RNA. |
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Storage |
at-20°C for one year |
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Kit Content |
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Component |
KD101-01 (6 rxns) |
KD101-02 (24 rxns) |
KD101-03 (96 rxns) |
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rRNA Probe (H/M/R) |
12 μl |
48 μl |
192 μl |
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E.coli RNase H |
6 μl |
24 μl |
96 μl |
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DNase I |
30 μl |
120 μl |
480 μl |
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5×Hybridization Buffer |
18 μl |
72 μl |
288 μl |
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10×RNase H Reaction Buffer |
12 μl |
48 μl |
192 μl |
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10×DNase I Reaction Buffer |
30 μl |
120 μl |
480 μl |
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RNase-free Water |
500 μl |
2×1 ml |
8×1 ml |
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Control qPCR |
Ribosomal RNA Primer Mix |
12 μl |
48 μl |
192 μl |
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Non-ribosomal RNA Primer Mix |
12 μl |
48 μl |
192 μl |
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