TECHNICAL SUPPORT
For all of our products, if you have any technical question, for example, on how to use the products, how to troubleshoot an issue, or obtain additional product information such as DNA sequence of a primer, please contact our tech team:
by email- techsupport@genebiosystems.com or by call-1-833-LabShop
For our customers who would like to get additional support in their research, we offer the following customized molecular biology service items. Please see below for details.
Our Customized Services
PCR
Difficult templates (GC-rich, secondary structures, high AT), long PCR, high-fidelity PCR, fast PCR, large number of templates, miRNA and other RNA reverse transcription PCR etc.
Cloning
PCR products, restriction endonuclease fragments, synthetic DNA constructs into cloning vectors and expression vectors for E. coli, yeast, insect and mammalian expression.
Site-directed mutagenesis
Point mutation, deletion and insertion mutation, multiple mutations, long inserts, GC-rich or simply difficult constructs
Library Construction
We have produced large-repertoire libraries utilizing our optimized cloning, and competent cells, and transformation approaches.
Site-directed mutagenesis
We can express and over-express your gene of interest in any of your expression systems of choice. We can also purify proteins and deliver the protein you need that meets your specifications.
Your Other Biology Needs
-including sequencing, troubleshooting, advice on cloning strategy, design, codon optimization, and ELISA etc.
References
Molecular products we played a key role in research, development and manufacturing:
1. DASL Rapid isothermal Amplification Assays, prototypes, technology patented, 2008-2018
2. Taqman, singleplex and 3plex realtime PCR Assays, commercialized, 2010-2018
3. Real-time PCR detection assays of viral pathogens, Sanger sequencing assays, commercialized, 2008-2010
4. Multiplexed microarray immunoassays, IVD, commercialized, 2006-2008.
5. Multiplexed molecular SNP assays, CFTR and personalized medicine,commercialized 2003-2006
6. recombinant human protein expression and purification, patented and published, antibody expression libraries and EST libraries, 1999-2003
Selected Molecular Biology Publications:
M. A. Carbone, N. Mackay, M. LING, D.E.C. Cole, C. Douglas, Rigat, B., Feigenbaum, A., Clarke, J.T.R., Haworth, J.C., Greenberg, C.R., Seargeant, L. and B. H. Robinson (1998). Amerindian pyruvate carboxylase deficiency is associated with two distinct missense mutations, American Journal of Human Genetics. Jun; 62(6): 1312-1319.
M. LING, McEachern, G., Seyda, A., MacKay, N., Scherer, S.W., Bratinova, S., Beatty, B., Giovannucci Uzielli, M.L., Robinson, B.H. (1998) Detection of a homozygous four base pair deletion in the protein X gene in a case of pyruvate dehydrogenase complex deficiency. Human Mol. Genetics., Mar; 7(3): 501-505.
M. LING, F. Merante, HC. Chen and B. H. Robinson (1997). The human mitochondrial elongation factor Tu gene: cDNA Sequence, genomic localization, genomic structure, and identification of a pseudogene. Gene, 197 (12), 325-336.
F. Merante, M. LING, HS. Chen and B. H. Robinson (1997). Cloning, characterization and localization of COX VIGL related genes. Genome 40 (3), 325-331.
M. LING and B. H. Robinson (1996). Rapid construction of three fragment recombinant DNAs by PCR: Application for gene targeting in Saccharomyces Cerevisiae. Analytical Biochemistry, 242, 155-158.
M. LING and B. Robinson (1995). A ONESTEP PCR method of site-directed mutagenesis of large gene cassettes with high efficiency, yield and fidelity. Analytical Biochemistry, 230, 167172.
M. LING, F. Merante and B. H. Robinson (1995). A very rapid and reliable DNA preparation method of screening a large number of yeast clones by polymerase chain reaction. Nucleic Acids Research, 23, 4924-4925.
M. LING, S. W. Allen and J. M. Wood (1994). Sequence analysis identifies the proline dehydrogenase and D1pyroline5carboxylate dehydrogenase domains of the multifunctional Escherichia coli PutA. Journal of Molecular Biology, 243, 950-956.