This product is designed for detecting mycoplasma DNA by TaqMan probe real-time PCR, which is used to qualitatively detect mycoplasma contamination in samples such as culture media, cell cultures, biological products, etc. This kit can detect a variety of mycoplasma, including Mycoplasma spp., Acholeplasma spp., Spiroplasma spp., etc. under the class Mollicutes. This kit was validated in accordance with EP 2.6.7 and JP G3 guidelines and requirements for mycoplasma detection.
TransDetect® qPCR Mycoplasma SuperMix in this product contains Taq hot start enzyme, specially optimized qPCR reaction buffer for mycoplasma detection, dNTPs, PCR enhancer and stabilizer. In addition, a dUTP/UDG system is introduced into this reaction mix, which can degrade U-containing ssDNA and dsDNA before reverse transcription, eliminating carry-over contamination caused by PCR products. Primer & Probe Mix is used to amplify mycoplasma sequences and Internal Control.
It is a duplex qPCR assay so that you can have an internal control to detect sample originated failures such as inhibitory substance in your DNA or other extraction failures. FAM channel detection of mycoplasma-specific amplification; The VIC channel detects the amplification of Internal Control. Internal Control can be added at the time of PCR reaction to exclude false-negative results due to PCR inhibitors in the sample; Internal control can also be added at the time of sample extraction to evaluate the extraction effect and rule out false negatives due to improper DNA extraction. This kit is used in conjunction with the MagicPure® 32 Mycoplasma DNA Kit (EH401-32) to efficiently extract mycoplasma DNA from samples, and the detection limit can reach 10 CFU/ml.
Features:
- Wide coverage of mycoplasma species: The validated mycoplasma detection covers 13 strains and plasmid DNA, with database matching capable of spanning at least 129 mycoplasma DNA sequences.
- High sensitivity validated mycoplasma strains meet the 95% detection requirement, with a detection limit as low as 10 CFU/mL; plasmid standards can reach 1 copy/μL
Table - detection limits for some of the species
| copies | /μl | |||||
|
Species |
Limit of Detection |
100 |
10 |
1 |
0.5 |
0.25 |
|
Mycoplasma gallisepticum |
5 copies |
2/2 |
2/2 |
20/20 |
8/8 |
8/8 |
|
Mycoplasma synoviae |
10 copies |
2/2 |
2/2 |
20/20 |
8/8 |
7/8 |
|
Mycoplasma arginini |
20 copies |
2/2 |
2/2 |
20/20 |
7/8 |
8/8 |
|
Spiroplasma citri |
10 copies |
2/2 |
2/2 |
20/20 |
8/8 |
7/8 |
|
Mycoplasma fermentans |
10 copies |
2/2 |
2/2 |
20/20 |
8/8 |
7/8 |
|
Acholeplasma laidlawii |
20 copies |
2/2 |
2/2 |
19/20 |
8/8 |
6/8 |
|
Mycoplasma hyorhinis |
20 copies |
2/2 |
2/2 |
20/20 |
7/8 |
4/8 |
|
Mycoplasma orale |
10 copies |
2/2 |
2/2 |
20/20 |
8/8 |
7/8 |
|
Mycoplasma pneumoniae |
10 copies |
2/2 |
2/2 |
20/20 |
8/8 |
7/8 |
|
Mycoplasma salivarium |
10 copies |
2/2 |
2/2 |
8/8 |
8/8 |
7/8 |
|
Malacoplasma penetrans |
10 copies |
2/2 |
2/2 |
8/8 |
8/8 |
8/8 |
|
Ureaplasma urealyticum |
20 copies |
2/2 |
2/2 |
8/8 |
6/8 |
6/8 |
|
Mycoplasma hominis |
10 copies |
2/2 |
2/2 |
8/8 |
8/8 |
7/8 |
3. Excellent specificity- Commonly found genomic sequences from nearly 20 types of bacteria, production cells, etc., do not interfere with the detection.
4. Excellent durability-product can withstand at least 15 freeze-thaw cycles without significant performance impact.
5. dUTP/UDG anti-contamination system, effectively preventing carry-over contamination of PCR products to ensure accurate data.
6. Broad instrument compatibility: Compatible with mainstream qPCR instruments, providing consistent results.
Storage
at -20℃ for two years in dark
Shipping
Dry ice (-70℃)
|
|
25rxn |
100rxn |
|
Component |
FM321-011 |
FM321-011 |
|
TransDetect® qPCR Mycoplasma SuperMix |
200 μl |
800 μl |
|
Primer&Probe Mix |
37μl |
150 μl |
|
Internal Control |
0.25 ml |
1 ml |
|
Positive Control |
100 μl |
400 μl |
|
Nuclease-free Water |
0.25 ml |
1 ml |