TransDetect® In Situ Fluorescein TUNEL Cell Apoptosis Detection Kit provides a precise, simple and low-toxicity way to detect and quantify apoptotic cell death at single cell level in cells and tissues. TdT-mediated dUTP Nick-End Labeling (TUNEL) reaction preferentially labels DNA strand breaks generated during apoptosis with fluorescein-labeled dUTP. The fluorescein labeled DNA can be detected and quantified by fluorescence microscopy or flow cytometry. This kit can be used to detect apoptosis in paraffin-embedded tissue sections, cryopreserved tissue sections, cells cultured on chamber slides, cell smear and cell suspensions.
Highlights
• Extremely low toxicity, labeling solution is free of commonly used highly-toxic cacodylate.
• High sensitivity, high specificity, optimal ratio of labeled and non-labeled substrates.
• Simple, one-step labeling by mixing TdT and labeling solution.
• Flexible, can be used in many assay systems.
Storage
TdT at -20℃ for one year, 1×Labeling Solution at -20℃ in dark for one year
Shipping
Dry ice (-70℃)
| Component | FA201-01 (25 rxns) | FA201-02 (50 rxns) |
| TdT | 50 μl | 100 μl |
| 1×Labeling Solution | 1.25 ml | 2×1.25 ml |
Li D Y , Shi X J , Li W , et al. Ischemic preconditioning and remote ischemic preconditioning provide combined protective effect against ischemia/reperfusion injury[J]. Life Sciences, 2016,150:76-80.