Size :50ul
Clone Number:28H4
Aliases:Histone H3.3, H3F3A, H3.3A, H3F3, PP781, AND, H3F3B, H3.3B
Product Type:Recombinant Antibody
Immunogen Species:Human
UniProt ID:P84243
Immunogen:A synthesized peptide
Raised in:
Species Reactivity:Human, Mouse
Tested Applications:ELISA, WB, ICC, FC; Recommended dilution: WB:1:500-1:5000, ICC:1:50-1:500
Background:Variant histone H3 which replaces conventional H3 in a wide range of nucleosomes in active genes. Constitutes the predominant form of histone H3 in non-dividing cells and is incorporated into chromatin independently of DNA synthesis. Deposited at sites of nucleosomal displacement throughout transcribed genes, suggesting that it represents an epigenetic imprint of transcriptionally active chromatin. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
Clonality:Monoclonal
Isotype:Rabbit IgG
Purification Method:Affinity-chromatography
Conjugate:Non-conjugated
Buffer:Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Form:Liquid
Stroage:Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Target Names:H3F3A
Research Areas:Epigenetics and Nuclear Signaling