Size: 10rxn.
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Description |
This kit takes advantage of proprietary assembly mix and homologous recombination. This kit can achieve directional cloning of PCR fragments that share 15-25 bp overlapping sequences into any linearized vector. •Fast: 15 minutes. •Broad: no restriction enzyme digestions. Can be cloned into any sites. •High efficiency: up to 95% cloning efficiency. •Seamless: no extra sequences introduced; up to 5 fragments assembly.
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Storage |
-20°C
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Kit Content |
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Component |
CU101-01 (10rxns) |
CU101-02 (20rxns) |
CU101-03 (60 rxns) |
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2×Assembly Mix |
50 μl |
100 μl |
3×100 μl |
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Linearized pUC19 Control Vector (10 ng/μl) |
3 μl |
6 μl |
18 μl |
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Control Insert (1 kb，20 ng/μl） |
3 μl |
6 μl |
18 μl |
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Please note: this kit does not include competent cells.
References:
| Literature | Journal | IF | Author | Date | Link |
|---|---|---|---|---|---|
| Switch of metabolic status: redirecting metabolic flux for acetoin production from glycerol by activating a silent glycerol catabolism pathway | METABOLIC ENGINEERING | 8.142 | Wang Y, et al. State Key Laboratory of Microbial M | 2017 Jan | The original link |
| A novel nematode effector suppresses plant immunity by activating host reactive oxygen species-scavenging system | new phytologist | 7.21 | Borong Lin, et al. | 2015 Oct | The original link |
| Phosphorylation-mediated Regulatory Networks in Mycelia of Pyricularia oryzae Revealed by Phosphoproteomic Analyses | MOLECULAR & CELLULAR PROTEOMICS | 6.5399 | Ruijin Wang,et al. | 2017Jul | The original link |
| The maize transcription factor EREB58 mediates the jasmonate-induced production of sesquiterpene volatiles. | Plant journal | 5.4679 | Li S, et al. Biotechnology Research Institute, Chi | 2015 Oct | The original link |
| Characterization of Pseudooxynicotine Amine Oxidase of Pseudomonas putida S16 that Is Crucial for Nicotine Degradation. | Scientific Reports | 5.2278 | Hu H, et al. State Key Laboratory of Microbial Met | 2015 Dec | The original link |