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Description |
pEASY ®-Blunt Simple Cloning Vector eliminates the multi-cloning sites of pEASY ® -Blunt Cloning Vector. It is designed for cloning and sequencing Pfu-amplified PCR products. •5 minutes fast ligation of Pfu-amplified PCR products. •Kanamycin and Ampicillin resistance genes for selection. •Easy blue/white selection. •SR primer and M13 forward primer for sequencing. •T7 promoter for in vitro transcription.
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Storage |
Competent Cell at -70°C for six months; others at -20°C for one year |
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Kit Content |
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Component |
CB111-01 |
CB111-02 |
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(20 rxns) |
(60 rxns) |
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pEASY ®-Blunt Simple Cloning Vector (10 ng/μl) |
20µl |
3×20µl |
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Control Template (5 ng/μl) |
5µl |
5µl |
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Control Primers (10 μM) |
5µl |
5µl |
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M13 Forward Primer (10 μM) |
50µl |
150µl |
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M13 Reverse Primer (10 μM) |
50µl |
150µl |
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SR Primer (10 μM) |
50µl |
150µl |
Please note: this kit does not include competent cells.
References:
| Literature | Journal | IF | Author | Date | Link |
|---|---|---|---|---|---|
| Phylogenetically diverse endozoic fungi in the South China Sea sponges and their potential in synthesizing bioactive natural products suggested by PKS gene and cytotoxic activity analysis | Fungal Diversity | 5.319 | Yu Z, et al. Marine Biotechnology Laboratory, Stat | 2013 Jan | The original link |
| Two Novel Fungal Phenolic UDP Glycosyltransferases from Absidia coerulea and Rhizopus japonicus | applied and environmental microbiology | 3.8229 | Kebo Xie,et al. | 2017Sep | The original link |
| RcRR1, a Rosa canina Type-A Response Regulator Gene, Is Involved in Cytokinin-Modulated Rhizoid Organogenesis | PLOS ONE | 3.73 | Bin Gao,et al. | 2013 Aug | The original link |
| Polymorphisms of Chicken TLR3 and 7 in Different Breeds | PLOS ONE | 3.73 | Wenke Ruan | 2015 Mar | The original link |
| Molecular cloning and functional characterization of multiple NADPH-cytochrome P450 reductases from Andrographis paniculata. | international journal of biological macromolecules | 3.6 | Huixin Lin et al National Resource Center for Chin | 2017 Apr | The original link |