Features:
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• 5 minutes fast ligation of Pfu-amplified PCR products. • Enhanced CMV promoter for higher protein yield. • CMV forward primer and TK polyA reverse primer for sequencing. • C-terminal myc tag for protein detection. • Neomycin resistance gene for stable cell line selection.
• M2 Expression Plasmid included as negative control. |
|
|
Storage |
Chemically Competent Cell at -70°C for six months; others at -20°C for nine months |
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Kit Content: |
|
Component |
CM211-01 (10 rxns) |
|
pEASY ®-Blunt M2 Expression Vector (15 ng/μl) |
10 µl |
|
M2 Expression Plasmid (Negative Control) (15 ng/μl) |
10 µl |
|
MControl Template (5 ng/μl) |
10 µl |
|
MControl Forward Primers (10 μM) |
10 µl |
|
MControl Reverse Primers (10 μM) |
10 µl |
|
CMV Forward Primer (10 μM) |
50 µl |
|
TK PolyA Reverse Primer (10 μM) |
50 µl |
|
Trans1-T1 Phage Resistant |
5×100 μl |
|
Chemically Competent Cell |
Papers citing the use of this product:
| Literature | Journal | IF | Author | Date | Link |
|---|---|---|---|---|---|
| The cellular distribution of Na+/H+ exchanger regulatory factor 1 is determined by the PDZ-I domain and regulates the malignant progression of breast cancer | Oncotarget | 5.008 | Du G, et al. Department of Biochemistry and Molecu | 2016 May | The original link |
| A Novel NHERF1 Mutation in Human Breast Cancer and Effects on Malignant Progression | anticancer research | 1.895 | Yang X, et al. Department of Biochemistry and Mole | 2017 Jan | The original link |
| A Novel NHERF1 Mutation in Human Breast Cancer Inactivates Inhibition by NHERF1 Protein in EGFR Signaling | Anticancer Research | 1.826 | GUIFANG DU, et al. | 2016 Mar | The original link |