Product Description
Hyperactive Universal CUT&Tag Assay Kit for Illumina is a kit developed specifically for the Illumina high-throughput sequencing platform to study protein-DNA interaction. CUT&Tag (Cleavage Under Target & Tagmentation) technology is a new method for studying protein-DNA interaction. By fusing Protein A/G with transposase, under the guidance of the antibody, it allows accurate targeting of the target protein and cutting of the DNA sequence near the target site. This kit optimizes the experimental reaction system and library construction process. Compared with the traditional ChIP-Seq, it has the advantages of low cell input, short experimental cycle, high signal-to-noise ratio, and good repeatability. It is especially suitable for such fields as early embryonic development, stem cells, tumors, and epigenetics. All the reagents in the kit have undergone rigorous quality control and function testing, to ensure the optimal stability and repeatability of library preparation.ut sequencing platform. All the reagents in the kit have undergone rigorous quality control and function testing, to ensure the optimal stability and repeatability of library preparation.
Applications
This product is intended for the protein-DNA interaction study in mammalian cells, and the input number of cells indicated is 60 - 100,000. Plant tissues, cells and others can also be used in this kit for related experiments through special treatments.