Features
• Fast: Amplified with fast & high-fidelity 2×TransStart® FastPfu PCR SuperMix; only 15 minutes for recombination.
• Flexible: Able to be cloned into any site to realize single-site/ multi-site, continuous/non-continuous mutagenesis.
• Efficient: >90% mutagenesis efficiency.
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Notes |
We suggest performing 25 cycles for PCR. For low yield PCR products, we suggest using up to 30 cycles. |
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Storage |
at -20°C for two years |
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Kit Content |
|
Component |
FM201-01 (10 rxns) |
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2×TransStart® FastPfu PCR SuperMix |
1 ml |
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DMT Enzyme (10 units/μl) |
30 μl |
|
2×Assembly Mix |
50 μl |
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DMT Chemically Competent Cell |
10×50 μl |
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ddH2O |
1 ml |
Papers citing this product:
| Literature | Journal | IF | Author | Date | Link |
|---|---|---|---|---|---|
| Regulation of Antimicrobial Peptides in Aedes aegypti Aag2 Cells | FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY | 5.218 | Zhang R, et al. Tsinghua-Peking Center for Life Sc | 2017 Feb | The original link |
| The cellular distribution of Na+/H+ exchanger regulatory factor 1 is determined by the PDZ-I domain and regulates the malignant progression of breast cancer | Oncotarget | 5.008 | Du G, et al. Department of Biochemistry and Molecu | 2016 May | The original link |
| Improving the catalytic efficiency of Bacillus pumilus CotA-laccase by site-directedmutagenesis | applied microbiology and biotechnology | 3.376 | Chen Y, et al. The Key Laboratory of Industrial Bi | 2016 Nob | The original link |
| Fascin phosphorylation sites combine to regulate esophageal squamous cancer cell behavior | Amino Acids | 3.173 | Fa?Min Zeng,et al. | 2017May | The original link |
| Identification and characterization of two types of amino acid-regulated acetyltransferases in actinobacteria | bioscience reports | 2.9059 | Yu-Xing Lu,et al. | 2017Jul | The original link |