DNase I (RNase-free)

GD201-01

Deoxyribonuclease I (DNase I) is an endonuclease that degrades double and single-strand DNA and chromatin. It functions by hydrolyzing phosphodiester linkages, producing mono and oligonucleotides with a 5'-phosphate and a 3'-hydroxyl group. Ribonuclease has been reduced to non-detectable levels. Its activity depends on Mg²⁺ or Mn²⁺ ion. DNase I with Mg²⁺ cuts randomly double strand DNA at any site, DNase I with Mn²⁺ cuts double strand DNA at the same site to form sticky-end with 1-2 nucleotide or form blunt-end.

Concentration: 3 units/μl

Storage
At -20˚C

Shipping
Dry ice (-70℃).

Product Contents

Component	GD201-01
DNase I (3 units/μl)	1500 units
10×DNase I Reaction Buffer	2×1 ml
200 mM EDTA	1 ml

Citations

Zhang G, Wang Z, Du Z, et al. mTOR Regulates Phase Separation of PGL Granules to ModulateTheir Autophagic Degradation[J] Cell, 2018, 174(6):1492-1506.

Liu Z Y , Yao X Q , Zhang Q , et al. Modulation of the Acetone/Butanol Ratio during Fermentation of Corn StoverDerived Hydrolysate by Clostridium beijerinckii Strain NCIMB 8052[J]. Applied & Environmental Microbiology, 2017, 83(7):AEM.03386-16.

Wang S , Wang C , Zhang X X , et al. Transcriptome de novo assembly and analysis of differentially expressed genes related to cytoplasmic male sterility in cabbage[J]. Plant Physiology & Biochemistry Ppb, 2016, 105:224-232.