VAHTS TGS DNA Library Prep Kit for Oxford Nanopore Technologies ONT
Size: 24 Rxns.
Keywords: DNA library construction; third generation library construction; TGS library construction; Nanopore sequencing; ONT sequencing
VAHTS TGS DNA Library Prep Kit for ONT is a ligation-based library construction kit suitable for Nanopore sequencing platform. Combined with the Barcode Adapter, it can perform single-sample or multi-sample analysis of 100 ng - 1.5 μg gDNA and amplicons. Build a database. This kit uses a directed evolution mutant enzyme with high catalytic activity and a carefully optimized buffer. It can directly perform Barcode Ligation without purification after end repair/damage repair/A-tail addition, and the experimental process is convenient. All reagents provided in the kit have undergone strict quality control and functional verification to ensure the stability and reproducibility of library construction.
Features:
1) The operation process is simple: steps of end repair + damage repair + adding A tail are completed in one tube, no purification is required, and Barcode connection can be performed directly
2) The time required for library construction is shortened: the minimum time required for the entire process of database construction can be <2 hours, which saves ≥30 minutes compared with the conventional process.
3) Compatible with various species you need to work with: compatible with animals, plants, microorganisms and other species
4) Excellent data quality: high read rate of long fragments, high data output, and good data quality
Better Data output and Quality
|
Species
|
Metric |
TS201
|
Supplier A
|
|
Human
|
Clean Total Reads
|
1,910,636
|
1,754,451
|
|
|
Clean Total bases
|
37,568,318,787
|
30,769,214,717
|
|
|
Clean Mean quality(Mean Q)
|
14.77
|
14.69
|
|
Plant
|
Clean Total Reads
|
3,398,731
|
3,494,821
|
|
|
Clean Total bases
|
32,590,018,744
|
31,687,125,980
|
|
|
Clean Mean quality(Mean Q)
|
12.86
|
12.77
|
|
Bacteria
|
Clean Total Reads
|
223,825
|
287,405
|
|
|
Clean Total bases
|
1,629,142,727
|
1,791,295,356
|
|
|
Clean Mean quality(Mean Q)
|
15.5 |
15.3 |
Better Longer Reads
|
Species
|
DNA Size
|
TS201
|
Supplier A |
|
Human |
5 - 50 kb |
63.63%
|
60.95%
|
|
|
50-100 kb
|
8.25%
|
6.57%
|
|
|
100-200 kb |
0.13%
|
0.11%
|
|
|
Total |
72.01%
|
67.63%
|
|
Plant |
5 - 50 kb |
39.63%
|
37.02%
|
|
|
50-100 kb
|
2.22%
|
2.10%
|
|
|
100-200 kb |
0.02%
|
0.02%
|
|
|
Total |
41.87% |
39.14% |
Kit composition
|
Component |
TS201-01 (24 rxns) |
|
End Prep & Repair Buffer |
36 µL
|
|
End Prep & Repair Master Enzyme Mix |
36 µL
|
|
Barcode Rapid Ligase Mix |
96 µL |
|
MP Adapter Fast Ligation Buffer |
240 µL
|
|
MP Adapter Fast Ligase |
120 µL |