FlyCut® HindIII

JH101-01

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Product Details

FlyCut^® HindIII is expressed and purified from E.coli that carries the recombinant HindIII gene. The molecular weight is 35.8kDa, with the recognition site at A^AGCTT. The reaction is conducted for 5-15 minutes at 37℃, and heat-inactivated at 65℃ for 20 minutes. This enzyme is not sensitive to dam, dcm or mammalian CpG methylation.

Recognition Site
5’...A↓AGCTT...3’
3’...TTCGA↑A...5’

Applications
Genomic DNA, plasmid DNA, PCR product

Storage
at -20°C  for two years

Shipping
Dry ice (-70 ℃)

Product Contents

Component	JH101-01	JH101-02
FlyCut® HindIII	5,000 units	2×5,000 units
10×FlyCut® Buffer	2×1 ml	3×1 ml
10×DNA Loading Buffer	1 ml	1 ml