FlyCut® EcoRV is expressed and purified from E.coli that carries the recombinant EcoRV gene. The molecular weight is 29.5 kDa, with the recognition site at GAT^ATC. The reaction is conducted for 5-15 minutes at 37℃, and heat-inactivated at 65℃ for 20 minutes. This enzyme is not sensitive to dam or dcm methylation, but sensitive to mammalian CpG methylation.
Recognition Site
5’...GAT↓ATC...3’
3’...CTA↑TAG...5’
Applications
Genomic DNA, plasmid DNA, PCR product
Storage
at -20°C for two years
Shipping
Dry ice (-70 ℃)
| Component | JE301-01 | JE301-02 |
| FlyCut® EcoRV | 2,000 units | 2×2,000 units |
| 10×FlyCut® Buffer | 500 μl | 1 ml |
| 10×DNA Loading Buffer | 1 ml | 1 ml |