TransScript® All-in-One First-Strand cDNA Synthesis SuperMix for qPCR (One-Step gDNA Removal )

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Tania Ridríguez Ramos
Great product, competitve price, fantastic customer service

The cDNA synthesis kit works great and it has a very competitive price. We use half a reaction and it still working great! Customer service is fantastic, they are always ready to help.

TransScript® All-in-One First-Strand cDNA Synthesis SuperMix for qPCR (One-Step gDNA Removal )

AT341-01
Regular price
¥38,800 JPY
Sale price
¥38,800 JPY
Regular price
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Unit price
per 
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20% Off NOW until March 31st as part of our Fiscal Year End Sale 2025! Buy this reverse transcription product, get 10% off towards PCR plates or 8-strip tubes, or sealing film.

Contact us or leave a comment in the notes section of your online order, and we'll adjust the price. **Shop now and save big!**

TransScript® All-in-One First-Strand cDNA Synthesis SuperMix for qPCR (One-Step gDNA Removal )-AT341

Product Details

The mastermix provides all the necessary components for cDNA synthesis from total RNA or mRNA. It is provided at 5× concentration and used at 1× concentration by adding gDNA remover, RNA and H2O. Simultaneous genomic DNA removal and cDNA synthesis are performed. After cDNA synthesis, gDNA remover and reverse transcriptase are inactivated by heating at 85℃ for 5 seconds. The resulting cDNA is suitable for qPCR (not for regular end-point PCR). qPCR mastermix is not included with product.

  • Simultaneous genomic DNA removal and cDNA synthesis. 
  • The optimal ratio of oligo(dT)18 primer to random primer(N9) for qPCR ready cDNA.
  • qPCR ready cDNA in 15 minutes.
  • cDNA up to 250 bp.

This mastermix synthesizes first-strand cDNA for qPCR, it does not contain the components for a qPCR reaction. 

PerfectStart® Universal Green qPCR SuperMix (Cat.#AQ602) sold separately from AT341.

For a true one-step cDNA synthesis and qPCR supermix (qRT-PCR), Please see our TransScript® II Green One-Step qRT-PCR SuperMix (Cat.#AQ311).

 

See manual for more details on AT341:

Applications

Multiple copy and low copy gene detection

Storage

at -20 ℃

Shipping

Ice/Gel packs (4℃)

 


Product contents


Component AT341-01 (50 rxns ) AT341-02 (100 rxns ) AT341-03 (500 rxns )
TransScript® All-in-One SuperMix for qPCR 200 μl 400 μl 5×400 μl
TransScript® All-in-One No-RT Control SuperMix for qPCR 20 μl 40 μl 200 μl
gDNA Remover 50 μl 100 μl 5×100 μl
RNase-free Water 1 ml 2×1 ml 2×5 ml

Citations

Han X , Wang R , Zhou Y , et al. Mapping the Mouse Cell Atlas by Microwell-Seq[J]. Cell, 2018, 172(5):1091-1107.e17.

Tian C , Zhang X , He J , et al. An organ boundary-enriched gene regulatory network uncovers regulatory hierarchies underlying axillary meristem initiation[J]. Molecular Systems Biology, 2014, 10(10):755-755.

Wang J , Zhang Y , Hou J , et al. Ube2s regulates Sox2 stability and mouse ES cell maintenance[J]. Cell death and differentiation, 2015, 23(2).

Zhang L , Zhao X , Zhang G , et al. Light-inducible genetic engineering and control of non-homologous end-joining in industrial eukaryotic microorganisms: LML 3.0 and OFN 1.0[J]. Scientific Reports, 2016, 6:20761.

Li P , Shi M L , Shen W L , et al. Coordinated regulation of IFITM1, 2 and 3 genes by an IFN-responsive enhancer through long-range chromatin interactions[J]. Biochimica et Biophysica Acta (BBA) - Gene Regulatory Mechanisms, 2017, 1860(8):885-893.

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