TransNGSTM rRNA Depletion Kit (Human-Mouse-Rat)

KD101

Description	TransNGS™ rRNA Depletion Kit (Human/Mouse/Rat) depletes ribosomal RNA from human/mouse/rat total RNA by RNase H digestion, while retains messenger RNA and non-coding RNA. The depleted ribosomal RNA contains both cytoplasmic ribosomal RNA (5S rRNA, 5.8S rRNA, 18S rRNA and 28S rRNA) and mitochodrial ribosomal RNA (12S rRNA and 16S rRNA). The resulting rRNA-depleted RNA is suitable for RNA-Seq, random-primed cDNA synthesis, or other downstream RNA analysis.
Features:	• Remove up to 99% ribosomal RNA from human/mouse/rat total RNA. • Control qPCR Primer Sets are provided to monitor the depletion efficiency of ribosomal RNA and the retention rate of non-ribosomal RNA.
Storage	at-20°C for one year
Kit Content

Component		KD101-01 (6 rxns)	KD101-02 (24 rxns)	KD101-03 (96 rxns)
rRNA Probe (H/M/R)		12 μl	48 μl	192 μl
E.coli RNase H		6 μl	24 μl	96 μl
DNase I		30 μl	120 μl	480 μl
5×Hybridization Buffer		18 μl	72 μl	288 μl
10×RNase H Reaction Buffer		12 μl	48 μl	192 μl
10×DNase I Reaction Buffer		30 μl	120 μl	480 μl
RNase-free Water		500 μl	2×1 ml	8×1 ml
Control qPCR	Ribosomal RNA Primer Mix	12 μl	48 μl	192 μl
Non-ribosomal RNA Primer Mix	12 μl	48 μl	192 μl