The purified transcript can be used for
RNAi experiments mediated by cationic liposome, calcium phosphate coprecipitation, electroporation, DEAE-dextran and microinjection.
Kit Composition
| Components | TR102-01 50 rxn | TR102-02 100 rxn |
| T7 Enzyme Mix | 50 μl | 100 μl |
| 10 × Transcription Buffer | 50 μl | 100 μl |
| 10 × Annealing Buffer | 250 μl | 500 μl |
| NTP Mix | 200 μl | 400 μl |
| DNase I | 25 μl | 50 μl |
| RNase T1 (100 U/μl) | 25 μl | 50 μl |
| RNase T1 Dilution Buffer | 300 μl | 600 μl |
| Control Template* | 5 μl | 10 μl |
| RNase-free H2O RNA Clean Beads |
5 ml 2 ml |
5 ml 4 ml |
Storage: Store all the components at -20℃.