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Introduction:
Introducing our Super Optimal Broth (SOB Medium), specifically formulated for the cultivation of E. coli in molecular biology applications. Fortified with vital nutrients, SOB Medium significantly enhances transfection efficiency, offering superior performance post-transfection.
Key Benefits:
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Nutrient-Rich Formula: Contains twice the amount of peptone compared to LB medium, providing an abundance of amino acids and polypeptides for optimal growth.
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High Density Culture: Infused with essential magnesium ions, promoting efficient growth in high-density cultures.
Advanced Composition:
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Tryptone: Supplies nitrogen, vitamins, minerals, and amino acids critical for robust cell growth.
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Yeast Extract: A rich source of B-group vitamins, essential for cellular health.
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Sodium Chloride and Potassium Chloride: Provide necessary ions to maintain cellular balance.
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Magnesium Sulfate: Delivers magnesium ions vital for various enzymatic reactions, including DNA replication.
Choose Super Optimal Broth (SOB Medium) for unparalleled results in your molecular biology experiments. Elevate your research with our premium formulation.
Approximate Formulation:
| Ingredients | g/liter |
| Tryptone |
20.0 |
| Yeast Extract |
5.0 |
| Sodium Chloride |
0.5 |
| Potassium Chloride |
0.186 |
| Magnesium Chloride |
0.95 |
| Final pH 7.0±0.2 at 25 ℃ |
Directions:
Suspend 26.6g of the medium in one liter of distilled water or deionized water. Mix well and heat with frequent agitation. Dispense into flasks or tubes. Sterilize in an autoclave at 121℃(15 lbs.) for 15 minutes.
DONT DELETE
For larger quantities, please contact us for details.