Each kit contains reagents for RNA extraction and purification, reverse transcription, and PCR amplification that have been optimized together in a simple workflow in one tube, which can save time, reduce experimental error, reduce pollution, and improve sensitivity. The kit can also be applied to one-step amplification of 2-1000 cells, and the cycle number should be reduced according to the number of cells.
| Components | P621-01 (200 rxn) |
| 2 x Reaction Mixa | 500 μl |
| RT/Taq enzymeb | 20 μl |
| Nuclease-free H2O | 1.25 ml x 2 |
a. Contains dNTP Mix, Mg2+ specific enhancer.
b. Contains Hiscript® II Reverse Transcriptase, RNase inhibitor, Champagne TaqTM DNA Polymerase
Store at -20℃.