Storage Conditions
Store at 2 ~ 8℃.
Product Introduction
RoomTemp TM Sample Lysis Kit is a simple and fast blood lysis kit at room temperature. This kit contains two components, Lysis Buffer and Stabilizing Buffer, among which Lysis Buffer contains special components that quickly destroy cell membrane proteins and membrane structure, which can be fully released The genomic DNA in the cell, Stabilizing Buffer is added with protective proteins and stabilizing factors, which can eliminate the inhibitory effect of the inhibitor in the lysed sample on the downstream qPCR and PCR reactions, so that the lysed DNA solution can be stored stably for a long time. Applicable blood sample types include Fresh blood, frozen blood and conventional anticoagulant blood (EDTA, citrate, sodium heparin, etc.). Genomic DNA can be released from whole blood samples after 3 minutes of lysis at room temperature. The lysed DNA solution can be directly used as a template ，Apply to Taqman ® probe method for SNP detection, qPCR probe method quantification, PCR amplification, etc. It can achieve the same effect as traditional genome extraction methods without complicated extraction operations. In addition to blood, this kit can also Compatible with pyrolysis FTA blood cards, oral swabs, plant tissues and other samples.
Product Advantages
Simple operation: 3 minutes at room temperature, no need for complex template extraction.
High lysis efficiency: The lysis reagent has the same effect as traditional kits for extracting genome.
Wide range of applications: Compatible with Taqman ® probe method for SNP detection, qPCR probe method quantification, PCR amplification and other sample compatibility: lysis of fresh blood, frozen blood, conventional anticoagulant blood, tissue homogenate, oral swabs, Various samples such as FTA card.