Size:100ug. Other sizes are also available. For further information, please contact us.
Research Areas:Others
Uniprot ID:Q9ICI2
Gene Names:N/A
Organism:Turnip mosaic virus (strain Japanese) (TuMV)
AA Sequence:TQQNRWMFEQLNGNLKAIAHCPSQLVTKHTVKGKCQMFDLYLKLHDEAREYFQPMLGQYQKSKLNREAYAKDLLKYATPIEAGNIDCDLFEKTVEIVVSDLRGYGFETCNYVTDENDIFEALNMKSAVGALYKGKKKDYFAEFTPEMKEEILKQSCERLFLGKMGVWNGSLKAELRPLEKVEANKTRTFTAAPLDTLLGGKVCVDDFNNQFYDHNLRAPWSVGMTKFYCGWDRLLESLPDGWVYCDADGSQFDSSLSPYLINAVLNIRLGFMEEWDIGEVMLRNLYTEIVYTPISTPDGTLVKKFKGNNSGQPSTVVDNTLMVILAVNYSLKKSGIPSELRDSIIRFFVNGDDLLLSVHPEYEYILDTMADNFRELGLKYTFDSRTREKGDLWFMSHQGHKREGIWIPKLEPERIVSILEWDRSKEPCHRLEAICAAMIESWGYDKLTHEIRKFYAWMIEQAPFSSLAQEGKAPYIAETALRKLYLDKEPAQEDLTHYLQAIFEDYEDGAEACVYHQ
Expression Region:2360-2876aa
Sequence Info:Partial
Source:E.coli
Tag Info:N-terminal 10xHis-tagged and C-terminal Myc-tagged
MW:66.6 kDa
Alternative Name(s):/
Relevance:Helper component proteinase: required for aphid transmission and also has proteolytic activity. Only cleaves a Gly-Gly dipeptide at its own C-terminus. Interacts with virions and aphid stylets. Acts as a suppressor of RNA-mediated gene silencing, also known as post-transcriptional gene silencing (PTGS), a mechanism of plant viral defense that limits the accumulation of viral RNAs. May have RNA-binding activity.
Reference:"The cylindrical inclusion gene of Turnip mosaic virus encodes a pathogenic determinant to the Brassica resistance gene TuRB01." Jenner C.E., Sanchez F., Nettleship S.B., Foster G.D., Ponz F., Walsh J.A. Mol. Plant Microbe Interact. 13:1102-1108(2000)
Purity:Greater than 85% as determined by SDS-PAGE.
Form:Liquid or Lyophilized powder
Buffer:If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution:We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20?/-80?. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Storage:The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20?/-80?. The shelf life of lyophilized form is 12 months at -20?/-80?.
Notes:Repeated freezing and thawing is not recommended. Store working aliquots at 4? for up to one week.
Function:
Involvement in disease:
Subcellular Location:
Protein Families:
Tissue Specificity:
Paythway:
HGNC Database Link:
UniGene Database Link:
KEGG Database Link:
STRING Database Link:
OMIM Database Link:
Lead Time Guidance:3-7 business days