Size:100ug. Other sizes are also available. Please contact us.
Research Areas:Immunology
Uniprot NO.:P01584
Uniprot Entry Name:IL1B_HUMAN
Gene Names:IL1B,IL1F2
Species:Homo sapiens (Human)
Source:E.Coli
Expression Region:117-269aa
Sequence:APVRSLNCTL RDSQQKSLVM SGPYELKALH LQGQDMEQQV VFSMSFVQGE ESNDKIP VAL GLKEKNLYLS CVLKDDKPTL QLESVDPKNY PKKKMEKRFV FNKIEINNKL EFESAQ FPNW YISTSQAENM PVFLGGTKGG QDITDFTMQF VSS
Protein Description:Full Length of Mature Protein
Tag Info:Tag-Free
Mol. Weight:17.3 kDa
Biological_Activity:Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine D10S cells is less than 1.0 pg/ml, corresponding to a specific activity of ? 1.0 × 109 IU/mg.
Purity:>97% as determined by SDS-PAGE and HPLC.
Endotoxin:Less than 1.0 EU/µg as determined by LAL method.
Form:Lyophilized powder
Buffer:Lyophilized from a 0.2 µm filtered PBS, pH 7.4
Reconstitution:We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20?/-80?. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Notes:Repeated freezing and thawing is not recommended. Store working aliquots at 4? for up to one week.
Alternative Name/ Alias:IL-1 beta,
Relevance:Produced by activated macrophages, IL-1 stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1 proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells. {ECO:0000269|PubMed:3920526}.
PubMed ID:6083565; 2989698; 3490654; 3493774; 2954882; 2635664; 11991722; 15815621; 15489334; 1919436; 3920526; 3281727; 1837236; 17431422; 3259176; 2585509; 2602367; 2372550; 2001363; 9062193; 20802483
Function:Potent proinflammatory cytokine. Initially discovered as the major endogenous pyrogen, induces prostaglandin synthesis, neutrophil influx and activation, T-cell activation and cytokine production, B-cell activation and antibody production, and fibroblast proliferation and collagen production. Promotes Th17 differentiation of T-cells.
Involvement in disease:
Subcellular Location:Cytoplasm, cytosol, Lysosome, Secreted, exosome, Secreted
Protein Families:IL-1 family
Tissue Specificity:Expressed in activated monocytes/macrophages (at protein level).
Paythway:MAPKsignalingpathway
HGNC Database Link:https://www.genenames.org/cgi-bin/gene_symbol_report?hgnc_id=HGNC:5992
UniGene Database Link:https://www.ncbi.nlm.nih.gov/UniGene/clust.cgi?ORG=Hs&CID=126256
KEGG Database Link:https://www.genome.jp/dbget-bin/www_bget?hsa:3553
STRING Database Link:https://string-db.org/network/9606.ENSP00000263341
OMIM Database Link:https://www.omim.org/entry/147720147720147720