Recombinant Human Desert hedgehog protein(DHH),partial (Active)

Recombinant Human Desert hedgehog protein(DHH),partial (Active)

CSB-AP000411HU
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Size:100ug. Other sizes are also available. Please contact us.

Research Areas:Stem Cells

Uniprot NO.:O43323

Uniprot Entry Name:DHH_HUMAN

Gene Names:DHH

Species:Homo sapiens (Human)

Source:E.Coli

Expression Region:24-198aa

Sequence:II+GPGRGPVG RRRYARKQLV PLLYKQFVPG VPERTLGASG PAEGRVARGS ERFRDLVPNY NPDIIFKDEE NSGADRLMTE RCKERVNALA IAVMNMWPGV RLRVTEGWDE DGHHAQDSLH YEGRALDITT SDRDRNKYGL LARLAVEAGF DWVYYESRNH VHVSVKADNS LAVRAGG

Protein Description:Partial

Tag Info:Tag-Free

Mol. Weight:19.9 kDa

Biological_Activity:Fully biologically active when compared to standard. The ED50 as determined by its ability to induce alkaline phosphatase production by C3H10T1/2(CCL-226) cells is 15-45 ?g/ml.

Purity:>96% as determined by SDS-PAGE and HPLC.

Endotoxin:Less than 1.0 EU/µg as determined by LAL method.

Form:Lyophilized powder

Buffer:Lyophilized from a 0.2 ?m filtered 10mM PB, pH 6.0, 300mM NaCl

Reconstitution:We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20?/-80?. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Notes:Repeated freezing and thawing is not recommended. Store working aliquots at 4? for up to one week.

Alternative Name/ Alias:DHH,

Relevance:Intercellular signal essential for a variety of patterning events during development. May function as a spermatocyte survival factor in the testes. Essential for testes development.

PubMed ID:15489334; 11017805; 19561611; 20519495; 15356051

Function:Intercellular signal essential for a variety of patterning events during development. May function as a spermatocyte survival factor in the testes. Essential for testes development.

Involvement in disease:Partial gonadal dysgenesis with minifascicular neuropathy 46,XY (PGD); 46,XY sex reversal 7 (SRXY7)

Subcellular Location:Desert hedgehog protein N-product: Cell membrane, Lipid-anchor, Extracellular side, Note=The N-terminal peptide remains associated with the cell surface, SUBCELLULAR LOCATION: Desert hedgehog protein C-product: Secreted, extracellular space

Protein Families:Hedgehog family

Tissue Specificity:

Paythway:Hedgehogsignalingpathway

HGNC Database Link:https://www.genenames.org/cgi-bin/gene_symbol_report?hgnc_id=HGNC:2865

UniGene Database Link:https://www.ncbi.nlm.nih.gov/UniGene/clust.cgi?ORG=Hs&CID=524382

KEGG Database Link:https://www.genome.jp/dbget-bin/www_bget?hsa:50846

STRING Database Link:https://string-db.org/network/9606.ENSP00000266991

OMIM Database Link:https://www.omim.org/entry/233420233420233420

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