Description
The FavorFilter Plasmid Extraction Midi Kit (Midiprep) is designed for rapid and efficient extraction of high-quality plasmid DNA. A special designed filter cartridge is allowed to remove the bacterial lysates without centrifugation step. Following a gravity-flow procedure, the plasmid DNA is bound to the resin, and the contaminants can be removed by wash buffer. The downstream applications of the FAFTE kit are transfection, in vitro transcription/translation, and all enzymatic modifications and more.
Features
- Time Saving: Remove bacterial lysates without centrifugation.
- High Purity: Equal to 2× CsCl gradient centrifugation method.
- Safety: Eliminate the use of phenol, chloroform, ethidium bromide, and cesium chloride; minimizing expose to hazardous materials.
Specifications
Format/Principle: Anion-exchange chromatography (Gravity-flow column)
Lysate Clarification: Filtration
Input Sample Size: Midi Prep: 60~120 ml of bacterial culture for high/low-copy high-copy number or low-copy number plasmids.
Plasmid or Constructs Range: 3 kbp~150 kbp
Binding Capacity: 650 µg/Midi Column
Applications
Transfection; microinjection; in vitro transcription; restriction enzyme digestion.
Procedure
In the process, the cell pellet is resuspended (PM1; RNase A contained), lysed (PM2), and neutralized (PM3). Next, the filter cartridge is used to remove bacterial lysates and obtain cleared sample mixture. Then, the plasmid DNA/buffer mixture bind to the ion exchange resin inside the PM Midi Column; the impurities are removed by PW Buffer. Finally, the purified plasmid DNA is eluted using high-salt PEL Buffer and precipitated with isopropanol for desalting.
Kit Composition
PEQ Buffer
PM1 Buffer
PM2 Buffer
PM3 Buffer
PW Buffer
PEL Buffer
RNase A (Lyophilized)
FavorFilter Midi
Cartridges and Columns
