Size:100ul
Host:Rabbit
Clonality:Polyclonal
Isotype:IgG
GeneName:WEE1
GeneID:7465
Uniprot:P30291
Specificity:Phospho-WEE1 (Ser642) Antibody detects endogenous levels of WEE1 only when phosphorylated at Ser642.
Immunogen:A synthesized peptide derived from human WEE1 around the phosphorylation site of Ser642.
Storage:Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.
Reactivity:Human,Mouse,Rat
Prediction:Pig,Rabbit,Dog
Application:WB,IF/ICC,ELISA(peptide)
Dilution:WB 1:1000-3000, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000
Conjugate:Unconjugated
RRID:AB_2841949
An Introduction to Blocking Peptides:A superior strategy for validation of antibody binding is the use of blocking peptides. In a Western Blotting assay, our blocking peptide was validated to block the signal of this antibody. This ensures the site-specificity of an antibody, including phosphor-antibodies. In the case of the phosphor- antibodies, the antibodies can and will only recognize the phosphorylated target protein at the corresponding site, not the non-phosphorylated target protein or target protein phosphorylated at a different site.
Cat# Blocking Peptide:DF8745-BP
Des# Blocking Peptide:Phospho-WEE1 (Ser642) Blocking peptide
Blocking Peptide Price:$549 CAD